Hirudinea Genomics Consortium a UCSD website

	Experimental Protocols Embryo Medium Embryonic Ganglia Luria Broth 50x TAE Antibody Staining Pond Water			Preparation of Artificial Pond Water Concentrate: 1 liter 2 liter 47.9 mM NaCl 2.8g 5.6g 0.67 mM KCl 0.05 g 0.1g 0.34 mM CA(NO3)2*H2O 0.08g 0.16g 0.1mM MgSO4*7H2O 0.025g 0.05g 100mM Hepes 23.8 g 47.6g pH to 7.4 Working solution: Dilute 1:100 with DI water = 189 ml per 5 gallon (18,900)