Hirudinea Genomics Consortium a UCSD website

	Experimental Protocols Embryo Medium Embryonic Ganglia Luria Broth 50x TAE Antibody Staining Pond Water			Preparation of Luria Broth 5X LB Luria Broth for E coli Propagation    100 g/L in double DI water.    LB Broth base Gibco catalog number 12780-029.    Aliquot in 100 ml bottles and autoclave.    Autoclave 30 minutes on liquid cycle.    Bottles standing in 1 inch of water with loose cap.    Autoclave tape on cap.